CA200
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CA200
Sigma
cAMP Enzyme Immunoassay Kit, Direct
sufficient for 96 assays
Description
| Application | Non-radioactive, competitive immunoassay for the quantitation of total cAMP in tissue and cell cultures. Buffer contains 0.1 N HCl that aids in the lysis of cells, inhibits endogenous phosphodiesterases and stabilizes the cyclic nucleotides. This kit uses a polyclonal antibody to cAMP to competitively bind cAMP or cAMP which has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cAMP present in the well. |
Properties
| usage | sufficient for 96 assays |
| shipped in | wet ice |
| storage temp. | −20°C |
Safety
| Hazard Codes | C |
| Risk Statements | 34 |
| Safety Statements | 26-36/37/39-45 |
| RIDADR | UN 3316 9/PG 3 |
Components
| Standard component | 0.1 M Hydrochloric Acid 1×30 mL |
| Acetic Anhydride 1×1 mL | |
| cAMP-Alkaline Phosphatase Conjugate 1×5 mL | |
| cAMP EIA Antibody Rabbit Anti-cAMP 1×5 mL | |
| 5 Cycle Logit-Log Paper 1×1 sheet | |
| Cyclic AMP Standard 1×0.5 mL | |
| Direct cAMP Assay Layout Sheet 1×1 sheet | |
| Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 each | |
| Neutralizing Reagent 1×6 mL | |
| p-Nitrophenyl Phosphate Substrate Solution 1×20 mL | |
| Plate Sealer 1 each | |
| Stop Solution 1×5 mL | |
| Triethylamine 1×2 mL | |
| Wash Buffer Concentrate 1×30 mL |
References
| reference | Chard, T. An Introduction to Radioimmunoassay and Related Techniques 4th ed., Amsterdam , (1990) |
| Tijssen, P. Practice and Theory of Enzyme Immunoassays Amsterdam , (1985) |
